Association of the single nucleotide polymorphism and the serum contents of interleukine-18 with ulcerative colitis

VernacularTitle:白细胞介素-18基因单核苷酸多态性及其血清含量与溃疡性结肠炎的相关性
Author: Yuebin DONG ; Hui XU
Publication Type:Journal Article
Keywords: Ulcerative colitis; Interleukin-18; Single nucleotide polymorphism
From: Chinese Journal of Digestion 2010;30(2):78-80
CountryChina
Language:Chinese
Abstract: Objective To estimate the association of the single nucleotide polymorphism (SNP) and the serum concentration of interleukin (IL)-18 with ulcerative colitis (UC) . Methods Samples collected from 50 patients with UC and 128 healthy controls were detected for SNPs of IL-18 gene including rs187238, rs5744228, rs360718 and rs360717 using polymerase chain reaction (PCR) and direct sequencing. Meanwhile, the serum level of IL-18 was measured. ResultsSerum concentration of IL-18 was higher in patients with UC than in healthy controls [(3029.9±111.7) pg/ml vs ( 133.2±39.4) pg/ml,(P<0.01)], rs187238 C, rs360718 C and rs360717 A were significantly related to UC (X~2=9.26, P<0.01). There was significant difference in frequencies of rs187238 C, rs360718 C and rs360717 A between patients with UC and healthy controls (X~2=9.26, P<0. 01). Serum concentration of IL-18 was higher in UC patients with C allele than in healthy controls (P<0.05).Conclusions There is correlation between SNP and serum level of IL-18 , which may involve in susceptibility to UC.