Empirical study on the apoptosis of gastric cancer cells induced by taxol
10.3760/cma.j.issn.1006-9801.2010.02.012
- VernacularTitle:紫杉醇诱导胃癌细胞凋亡的实验研究
- Author:
Haocheng LONG
;
Xia GAO
;
Zhijian PAN
;
Chunzhou CHEN
;
Jianping GONG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Apoptosis;
Cell cycle;
Taxol
- From:
Cancer Research and Clinic
2010;22(2):108-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate apoptosis of gastric cancer cells induced by taxol,and its specific apoptotic cell cycle phase. Methods The apoptosis rate of gastric cancer cell line MKN-28 induced by taxol was detected with Sub-G1 method.The specific apoptotic cell cycle phase Was detected with API and PSC method.The sensitivity of 20 cases clinic gastric cancer specimens induced by taxol was detected with the method MTT.Results With the Sub-G1 method the MKN-28 cells were induced by 10 μg/ml taxol,after 10 h,the apoptosis rate reached its top apex;with the API method and the PSC method,the specific apoptosts took place in G_2/M phase;the chemotherapy sensitivity of 16 cases out of 20 cases clinic gastnc cancer specimens exceed 50%with the method MTT. Conclusion Taxol could induce gastric cancer cells to aimptosis and the apoptosis takes place in G_2/M phase;Taxol is sensitive to clinic gastric cancer speclmens.
