Photochemically induced incomplete spinal cord injury in cat
10.3760/cma.j.issn.0254-1424.2010.03.001
- VernacularTitle:光化学法诱导猫不完全脊髓损伤动物模型的建立
- Author:
Yizhao WANG
;
Jiang XU
;
Tao XU
;
Zhengyu FANG
;
Qi XU
;
Xiaolin HUANG
- Publication Type:Journal Article
- Keywords:
Photochemistry;
Spinal cord injury;
Cat
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2010;32(3):161-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential of creating a cat model of spinal cord infarction initiated by a photochemical reaction in terms of the neuroethology, motor evoked potential, and morphological outcomes. Meth-ods Fifteen cats were divided into three groups at random. T_13 in the spinal cord was photochemically irradiated for 40 min, 60 min or 80 min in different groups. A photochemically-induced infarction was produced by intravenous in-jection of rose Bengal (35 mg/kg) combined with immediate cold light irradiation (3000 klx) of the spinal cord.Neuroethology changes were observed every day after the surgery far 21 days, and morphological changes were exam-ined at day 21, before which the motor evoked potential was examined and compared with measurements taken before injury. Results The spinal cord infarctions induced by intravenous injection of rose Bengal plus cold light irradia-tion for 40 min were stable by day 8, while the other two groups were stable by day 12. The size of the infarction area in the spinal cord was stable, while the neuroethology, electrophysiological and histopathological changes in the cats were significant. Conclusions All of the cats demonstrated decreased functional mobility after photochemically in-duced thrombosis of the spinal cord, with corresponding pathomorphological and electrophysiologic changes. The model of infarction was stable and reliable.
