Changes in synaptophysin and growth associated protein-43 mRNA around a cerebral infarction after mobility training
10.3760/cma.j.issn.0254-1424.2010.03.002
- VernacularTitle:运动训练对大鼠脑梗死灶周突触素mRNA及生长相关蛋白mRNA水平的影响
- Author:
Laixun TAN
;
Xinguo YE
;
Zhichao LIU
;
Shenggang SUN
- Publication Type:Journal Article
- Keywords:
Synaptophysin;
Growth associated protein-43;
Motor skill;
Cerebral infarction
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2010;32(3):166-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of mobility training on mobility and the mRNA levels of both synaptophysin and growth associated protein 43 (GAP-43) in the region around an infarction in rats with acute cerebral infarction. Methods Models of cerebral infarction were created in 100 rats through middle cerebral artery occlusion. They were then randomly divided into training and control groups. The motor skill of the rats was examined using a beam walking test. The mRNA levels of both synaptophysin and GAP-43 in the region around the infarction were observed at the 1st, 3rd, 7th, 14th and 28th days after model-creation using a semi-quantitive reverse transcrip-tion polymerase chain reaction. Results The rats' mobility scores increased with training, and significant differ-ences were observed between the average scores of the two groups at the 3rd, 7th and 14th days. The scores were higher in the training group. The mRNA levels of both synaptophysin and GAP-43 in the region around the infarction increased significantly from the 1st to the 3rd and 7th days. Synaptophysin mRNA levels were significantly higher in the trained group at each time point, but the levels of GAP-43 mRNA were significantly higher in the trained group only on the 3rd and 7th days. Conclusions Motor skill and the mRNA levels of synaptophysin and GAP-43 in the region around an infarction can be increased by motor skill training, at least in rats with model acute cerebral infarc-tion.
