Role of interleukin-17 in glomerulosclerosis secondary to adriamycin-induced nephropathy in rats

VernacularTitle:白细胞介素-17在阿霉素肾病大鼠肾小球硬化中的作用
Author: Li WANG ; Wei ZHANG ; Qiu LI
Publication Type:Journal Article
Keywords: Interleukin-17; Adriamycin; Glomerulosclerosis; Nephrotie syndrome
From: Chinese Journal of Microbiology and Immunology 2010;30(3):245-249
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of interleukin-17 (IL-17) in glomerulosclerosis secondary to adriamycin-induced nephropathy in rats. Methods Male Sprague-Dawley rats were divided into normal control group and adriarnycin-induced nephropathy group(model group). Eight rats were sacrificed at 2nd week, 4th week, 8th week, 12th week respectively. Histopathological changes of renal tissue were observed under light microscope and glomerulosclerosis index was evaluated. The expression of IL-17 was measured by RT-PCR, ELISA and immunohistochemistry. The expression of IL-1β was detected by ELISA. Results Compared with control group, the expressions of IL-17 mRNA, IL-17 and IL-1β protein were higher in model group of the 8th week (P=0.041, P=0.000 and P=0.007 respectively), even they were further higher in model group of the 12th week than that in the control group (all P=0.000). In model group of the 12th week, the level of IL-17 protein had positive correlation with IL-1β and glomerulosclerosis index (P=0.037, P=0.021 respectively). Conclusion IL-17 expression is elevated and may contribute to glomerulosclerosis in adriamycin-induced nephropathy.