Th1/Th2 cytokines response to purified rabies vaccine for human use

VernacularTitle:接种纯化人用狂犬病疫苗对Th1/Th2类细胞因子谱的影响
Author: Fanglan LIU ; Jinyan LIN ; Ruilin MENG ; Qiong HUANG ; Xiaohua TAN
Publication Type:Journal Article
Keywords: RV; Lymphacytes; Th1/Th2 cytokines; Cytometric bead array
From: Chinese Journal of Microbiology and Immunology 2010;30(3):263-266
CountryChina
Language:Chinese
Abstract: Objective To study the effects of purified rabies vaccine for human use (RV) on specific Th1/Th2 cytokines in human. Methods Twenty cases were injected intramuscularly with 5 full doses of RV. PBMCs were isolated from the blood sample collected at day 0, 14, 45 after the RV inoculation. Neutralizing antibody was determined by ELISA, and the proliferation of lymphocyte by in vitro test. The levels of RV specific IFN-γ, TNF, IL-2,IL-4, IL-5, IL-10 in the culture supernatants were detected by cytometric bead array (CBA). Results The neutralizing antibody was tested positive in 19 cases 45 days after inoculation and 1 case after 60 days, with the positive rate reaching 100%. After stimulation with RV, the lymphocyte transformation index at day 14, 45 in cases were significantly higher than those day of 0 (P< 0.05), and similar results were confirmed with IFN-γ, IL-2, IL-4, IL-5 tested by CBA (P<0.05). Condusion The RV could induce humoral and antigen-specific cellular immune responses in human, tested by showing good protective effect on rabies virus.