Sequence variation in the env region of SHIV-XJ02170 during in vivo passaging
10.3760/cma.j.issn.0254-5101.2010.05.013
- VernacularTitle:SHIV-XJ02170在中国恒河猴体内传代过程中env基因变异研究
- Author:
Qiang LIU
;
Yue LI
;
Guibo YANG
;
Qiang WEI
;
Chuan QIN
;
Yiming SHAO
- Publication Type:Journal Article
- Keywords:
SHIV;
Passage;
Env;
Variation
- From:
Chinese Journal of Microbiology and Immunology
2010;30(5):443-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the variation in the Env region of SHIV-XJ02170 during passaging in Chinese origin Rhesus Macaques.Methods Fragments of the SHIV-XJ02170 gp160 and gp120 gene were amplified by PCR and RT-PCR separately from the blood samples of SHIV-XJ02170 infected animals at the peak viral load time point.Purified RT-PCR product was ligated into T easy vector and transformed into JM109 competent cells,18 clones were selected by PCR method and sequenced by ABI 3730DNA sequencers.The gene distances(divergence,diversity)were calculated using DISTANCE.Results In all,the SHTV-XJ02170 gp120 gene evolved forward along the virus passaging.It could be found that viral divergence from the founder strain serially enhanced during in vivo passaging,but in the early phase of each passage,SHIV-XJ02170 gp120 gene evolved toward ancestral state upon transmission to a new host.All of the SHIV-XJ02170 strains had V3 loop central motif(GPGQ)and were predicted to be using CCR5 on the basis of the critical amino acids within V3 loop.Conclusion There was significant increase in the genetic distance during serial passaging,and SHIV-XJ02170 gp120 gene evolved forward along passaging.This could partly explain why the virus infectivity was enhanced during in vivo passaging.
