Investigation on the genotyping of Acinetobacter baumannii by DiversiLab system
10.3760/cma.j.issn.1009-9158.2010.05.009
- VernacularTitle:DiversiLab系统鲍曼不动杆菌基因分型可行性研究
- Author:
Yanyan QU
;
Wenfei WANG
;
Hua ZHOU
;
Yiqi FU
;
Yunsong YU
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Polymerase chain reaction;
Repetitive sequences,nucleic acid;
Electrophoresis,gel pulsed-field;
Bacterial typing techniques;
Reproducibility of results
- From:
Chinese Journal of Laboratory Medicine
2010;33(5):425-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the usefulness of DiversiLab system for genotyping of Acinetobacter baumannii.Methods Fifty-eight non-duplicated clinical Acinetobacter baumannii isolated from 15 cities in China in 2005 were typed by rep-PCR-based DiversiLab system.The results were compared with those of PFGE and multilocus sequence typing. Simpson's index of diversity was used to compare the discriminatory power among DiversiLab system, PFGE and MLST.Results Fifty-eight Acinetobacter baumannii isolates were differentiated into 5 clusters and 25 unique types by DiversiLab system. MLST identified 35 distinct sequence types, which fell into one clone complex of CC22 and 35 singletons, while PFGE resolved 5 pulsotypes and 34 unique types.Simpson's diversity indices for DiversiLab system, MLST and PFGE were O.876, O.944 and 0.961, respectively.Conclusions The discriminatory power of DiversiLab system is lower than that of PFGE and MLST.But as a simple, fast and reproducible typing method, it could be used as a first-line typing tool for the analysis of a large number of isolates.
