A novel potential neutralizing epitope of hepatitis E virus
10.3760/cma.j.issn.0254-5101.2010.04.018
- VernacularTitle:戊型肝炎病毒新潜在中和性表位的发现
- Author:
Zizheng ZHENG
;
Ming TANG
;
Ji MIAO
;
Min ZHAO
;
Hui HUANG
;
Jingxian LI
;
Hai YU
;
Shaowei LI
;
Jun ZHANG
;
Ningshao XIA
- Publication Type:Journal Article
- Keywords:
Neutralizing epitope;
Non-immunodominant epitope;
Hepatitis E virus;
Capoid
- From:
Chinese Journal of Microbiology and Immunology
2010;30(4):360-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To search for the neutralizing epitopos on hepatitis E virus (HEV) capsid besides the known neutralizing epitope (aa459-606). Methods By analysis of several strains of monoclonal antibodies against HEV capsid and their recognized epitopes, the neutralizing activity of epitope (aa394-458) at N-terminus was compared with that of an immunodominant neutralizing epitope (an459-606). Re-suits The research showed a novel potential neutralizing epitope in aa423-437 of HEV ORF2 though detec-ting and comparing the characteristics of several antibodies and corresponding determinations. The epitope is a linear non-immunodominant epitope which is different from the other neutralizing epitope in aa459-606.And the amino acids sequence of this novel epitope is conservative. Conclusion ORF2 aa423-437 is a no-vel potential neutralizing leaner epitope of HEV. It is believed that the present work adds fundamental knowl-edge to our understanding of HEV capsid domain and contributes to the prevention and control of this dis-ease.
