Detection for msr gene in biovar Parvo and biovar T960 of Ureaplasma urealyticum
10.3760/cma.j.issn.0254-5101.2010.04.021
- VernacularTitle:解脲脲原体Parvo和T960生物群msr基因的检测
- Author:
Tinglu YE
;
Chun LU
;
Chuanjie CHEN
;
Guoxing ZHU
;
Han MA
;
Rongbiao LU
;
Wei LAI
- Publication Type:Journal Article
- Keywords:
Ureaplasma urealyticum;
Erythromycin;
Antimicrobial susceptibility testing;
msr gene
- From:
Chinese Journal of Microbiology and Immunology
2010;30(4):377-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the msr gene which confers resistance to erythromycin, and ana-lyze its distributing difference between the two biovars of Ureaplasma urealyticum. Methods Broth dilution method was used to determine the minimum inhibitory concentrations (MIC) to erythromycin among 72 U. urealyticum clinical isolates. The msrA, msrB, msrC and msrD genes detection and biotyping of U. urea-lyticum were conducted using PCR. Results The MICs of 72 U. urealyticum isolates to erythromycin ranged from ≤0. 125 μg/ml to ≥128 μg/ml. MIC_(50) was 32 μg/ml and MIC_(50) was ≥128 μg/ml. Biotyping showed that biovar Parvo had 51 strains (51/72, 70.83%) and biovar T960 had 21 (21/72, 29.17%) strains.The msrA, msrB, msrC and msrD genes were obtained in 1, 12, 0 and 24 strains, respectively, with five strains carrying the msrB and msrD genes, and one strain carrying the msrA, msrB and msrD genes. There was no resistance difference to erythromycin between the two biovars when the MIC≥8 μg/ml was considered resistance to eryt hromycin. But the msrB gene was predominantly detected in biovar T960. Conclusion U. urealyticum clinical isolates harbeur the msrA, msrB and msrD genes, and the predominantly detected msrB gene is of biovar T960.
