Effect of FTY720 on human hepatocellular carcinoma cell line HepG-2 of invasion ability
10.3760/cma.j.issn.1006-9801.2010.05.010
- VernacularTitle:FTY720对人类肝癌细胞HepG-2侵袭能力的影响
- Author:
Yun LIU
;
Shili ZANG
;
Haoliang ZHAO
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinase-2;
Matrix metalloproteinase-9;
Neoplasm invasiveness;
FTY720
- From:
Cancer Research and Clinic
2010;22(5):315-318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of FTY720 on human hepatocellular carcinoma cell line HepG-2 of invasion ability. Methods HepG-2 cells were cultured,and divided into 4 groups,namely control and FTY720 (0.05 g/ml,0.10 g/ml,0.15 g/ml) groups.The cell invasion ability was observed by the cell invasion assay.mRNA expression of MMP-2,MMP-9 was measured by RT-PCR.MMP-2,MMP-9 protein expression was measured by ELISA method.Results The invasive ability of cancer cells in each group significantly weaken,with the FTY720 concentration increased.The number of control group was (110±3)(P<0.05),the number of O.05 g/ml FRY720 treatment group cells was (74±4),while the number of cells of O.10 g/ml,0.15 g/ml the drug treated group,Was (42±3),(25±5)(P<0.01).Compared with control group,the MMP-2,MMP-9 expression of different concentrations of FTY720 groups decreased significantly (P<0.05).As the expression of FTY720 concentration increased,the MMP-2,MMP-9 expression decreased (P<0.01).Compared with control group,the MMP-2,MMP-9 protein expression of different concentrations of FTY720 groups decreased significantly (P<0.05), but the protein expression and concentration of FTY720 is inversely proportional in a certain range. Conclusion The invasive ability of HepG-2 cells can be inhibited by FTY720,and with FTY720 concentration increased,the invasive ability decreased gradually.One of the mechanisms may be related to reduce the MMP-2,MMP-9 protein and gene expression.
