Expression and biological significance of Runx2 in dedifferentiated chondrosarcoma cell line

VernacularTitle:Runx2在去分化软骨肉瘤细胞系中的表达及其意义
Author: Xinchang LU ; Xiaodong TANG ; Wei GUO ; Guowen WANG
Publication Type:Journal Article
Keywords: Runx2; Dedifferentiated chondrosarcoma; NDCS-1
From: Cancer Research and Clinic 2010;22(4):231-234
CountryChina
Language:Chinese
Abstract: Objective To detect the different expression of Runx2 in dedifferentiated chondrosarcoma and conventional chondrosarcoma, and to investigate the role of Runx2 in the occurrence and development of dedifferentiated chondrosarcoma. Methods Dedifferentiated chondrosarcoma cell line NDCS-1 and normal chondrosarcoma cell line SW1353 were cultured, then mRNA and total cellular protein were extracted.RT-PCR Western blotting, and immunocytochemistry were used to detect the expression of Runx2.Immunohistochemistry was used to test Runx2's expression in the dedifferentiated chondrosarcoma specim ens that confirmed by pathology. Results Runx2 was high expression in dedifferentiated chondrosarcoma cell line and high-grade component of dedifferentiated chondrosarcoma tissues. Conclusion The high expression of Runx2 in dedifferentiated chondrosarcoma is involved in the occurrence and development of dedifferentiated chondrosarcoma.