Partial genome molecular characteristics of Getah virus newly isolated in China
10.3760/cma.j.issn.0254-5101.2010.05.003
- VernacularTitle:我国新分离盖塔病毒的部分基因组分子特征研究
- Author:
Weixin CHEN
;
Huanyu WANG
;
Shihong FU
;
Minghua LI
;
Guifang LIU
;
Hongyue JIANG
;
Lihua WANG
;
Haiyan WANG
;
Zhiyu WANG
;
Guodong LIANG
- Publication Type:Journal Article
- Keywords:
Getah virus;
Capsid gene;
E2 protein gene;
3' untranslated region;
Nucleotide sequence
- From:
Chinese Journal of Microbiology and Immunology
2010;30(5):399-404
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the genome molecular characteristics of Getah virus(DY0824)which isolated in Shandong province,2008 by molecular biology methods.Methods Reverse transcriptasepolymerase chain reaction(RT-PCR)was used to amplify the structural gene and 3'UTR fragments then the RT-PCR products were inserted into PGEM-T easy to be sequenced.Computer software was used to analyze the nucleotide and deduced amino acid sequence,and draw phylogenetic trees,including Clustal X1.83 and MegaAlign and Mega4.Results The capsid protein of DY0824 consists of 804 nucleotides,encoding 268 amino acids and the full-length of E2 protein is 1266 nucleotides,encoding 422 amino acids.The nucleotide homology of the capsid protein and the E2 protein with other strains were 95.4%-99.9%and 94.8%-99.5%,and the amino acid were 97.4%-100%and 97.6%-100%.The 3'UTR of the virus include 401 nucleotides and there are three repeat sequence elements.Conclusion Compared with the prototype virus,the Getah virus isolated in Shandong province had 7 amino acid differences in capsid protein genes and 10 amino acid differences in E protein genes.The 3'UTR region had multi-nucleotide changes.
