Isolation and identification of the viral pathogen in an incident with crowd fever of adults

VernacularTitle:一起成人群体发热患者的病毒分离与鉴定
Author: Yan HU ; Bingke BAI ; Boan LI ; Min ZHAO ; Jun HOU ; Honghui SHEN ; Wei HONG ; Yuanli MAO ; Panyong MAO
Publication Type:Journal Article
Keywords: Crowd fever; Virus isolation; Identification; Adenovirus
From: Chinese Journal of Microbiology and Immunology 2010;30(5):416-419
CountryChina
Language:Chinese
Abstract: Objective To isolate and identify the causative agent in an incident with crowd fever of adults in Henan province.Methods The cells was inoculated by the throat swabs of the patients and followed by neutralization assay and other molecular methods.Then indirect immunofluorescence assay was performed to detect the specific IgM/IgG antibodies against virus in the serum of the patients.Results We have isolated 2 strain adenoviruses from 10 swab samples,which were both identified as Ad11 by sequence analysis and neutralization test.6 of 10 samples were positive fer IgM specific for adenovirus and 3 positive for IgG.The remaining sample was negative for both.Conclusion The causative agent in this incident with crowd fever of adults was Ad11 in group B.