The effect of electromagnetic field stimulation on the mRNA expression of fibroblast growth factor and it's receptor in bone marrow mesenchymal stem cells in vitro
10.3760/cma.j.issn.0254-1424.2010.04.004
- VernacularTitle:电磁场对大鼠骨髓间充质干细胞成纤维细胞生长因子-2和成纤维细胞生长因子受体-2mRNA表达的影响
- Author:
Chaoxu LIU
;
Yong YANG
;
Jizhe YU
;
Guizhen LI
;
Yadong ZHENG
;
Fei PENG
;
Wenchun ZHAO
;
Hua WU
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Fibroblast growth factor-2;
Reverse transcription-polymerase chain reaction;
Electromagnetic fields
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2010;32(4):253-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of an electromagnetic field (EMF) on the expression of fibro-blast growth factor (FGF-2) and it' s receptor (FGFR-2) mRNA in rat bone marrow mesenchymal stem cells (BMSCs) in vitro. Methods Rat BMSCs were isolated and cultured in vitro. The subcultured cells were divided into different groups to be EMF stimulated at 1.0 mT. The expression of FGF-2 and FGFR-2 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results Different frequencies and durations of 1.0 mT EMF exposure induced FGF-2 and FGFR-2 mRNA expression in comparison to blank controls. The expression of FGF-2 mRNA reached a peak after stimulation at 15 Hz for 10 min, 50 Hz for 60 min and 75 Hz for 30 min. And the expression of FGFR-2 mRNA reached a peak after 30 minutes at all frequencies. At 1.0 mT with 30 min exposure, the expression of FGF-2 mRNA peaked after 50 Hz stimulation, and the expression of FGFR-2 mRNA peaked after stimulation at 75 Hz. Conclusions Moderate EMF stimulation can significantly increase the expression of FGF-2 and FGFR-2 mRNA in rat BMSCs in vitro.
