The effects of electromagnetic fields on osteoporosis in ovariectomized mice
10.3760/cma.j.issn.0254-1424.2010.04.005
- VernacularTitle:电磁场防治去卵巢小鼠骨质疏松的实验研究
- Author:
Chaoxu LIU
;
Jizhe YU
;
Yong YANG
;
Yadong ZHENG
;
Fei PENG
;
Guizhen LI
;
Wenchun ZHAO
;
Hua WU
- Publication Type:Journal Article
- Keywords:
Electromagnetic fields;
Alkaline phosphatase;
Bone gamma-carboxyglutamic-aeid-containing proteins;
Estradiol;
Bone trabeculae
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2010;32(4):258-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of electromagnetic field (EMF) exposure on osteoporosis in ovariectomized mice. Methods Sixty 8-week-old female Kunming mice were divided into four groups at random: a sham operation group (group A), an ovariectomized group (group B), an EMF and ovariectomized group (group C) and a nilestriol and ovariectomized group (group D). Bilateral ovariectomies were performed on all mice except those in group A. The mice of group C were exposured to a 15 Hz, 1.0 mT electromagnetic field. The mice of group D were given at nilestriol 1.5 mg/kg/week. The bone mineral density (BMD) of the lumbar vertebrae was measured before the mice were sacrificed at the 12th week. Blood specimens were collected every two weeks to measure the ac-tivity of alkaline phosphatase (ALP) and the concentration of bone gamma-carboxyglutamic-acid-containing proteins (BGP), calcium and estradiol in the serum. Histological sections were taken to examine and analyze the changes in bone trabeculae in the lumbar vertebrae after 6 and 12 weeks. Results EMF at 15 Hz and 1.0 mT intensity signifi-cantly increased the activity of ALP and the concentrations of BGP and calcium in the serum. In addition, the absorp-tion of bone trabeculae in the lumbar vertebrae was significantly restrained. Conclusions EMF at 15 Hz and 1.0 mT can restrain the development of osteoporosis in ovariectomized mice.
