TSLP promotes lung Inflammation via activating dendritic cells in OVA-induced mice asthmatic model
10.3760/cma.j.issn.0254-5101.2010.04.003
- VernacularTitle:哮喘小鼠气道上皮TSLP表达及激活DCs加重气道炎症的研究
- Author:
Yanli LI
;
Hongjia LI
;
Huijuan QI
;
Rong WANG
;
Feng JI
;
Junqing HAO
;
Wenxiang BI
;
Liang DONG
- Publication Type:Journal Article
- Keywords:
Airway epithelium;
TSLP;
Dendritic cell;
Bronchial asthma
- From:
Chinese Journal of Microbiology and Immunology
2010;30(4):303-308
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of thymic stromal lymphopoietin(TSLP) and the activation of DCs in OVA-induced murine asthma model, and investigate the effects and underlying mecha-nisms of TSLP on lung inflammation. Methods Thirty BALB/c mice were randomly divided into control group, OVA group and TSLP neutralizing antibody treated group. The asthma model was evaluated by airway responsiveness and histological analysis of lung tissues ; The levels of TSLP mRNA in lungs were determined by quantitative real-time PCR; The expression of TSLP in lungs were determined by immunohistochemistry and Western blot; The expression of CD40, CD80, CD86 in BALF was detected by FACS. Results Both the histological analysis of lung tissues and the airway responsiveness were all consistent with the characteris-tic of murine asthma model. The expression of TSLP and TSLP mRNA in the OVA group was significantly in-creased compared with blank group. The expression of CD40, CD80, CD86 in BALF from OVA group was increased significantly compared with the control group. Furthermore, treating mice with TSLP neutralizing antibody reduced the expression of CD40, CD80, CD86 on dendritic cells, and IL-4, IL-5, IL-13 in the OVA group. Conclusion Our study indicate that TSLP was highly expressed in the bronchial epithelia of murine asthma model, via upregulation of CD40, CD80, CD86, induce DCs to active CD4~+ T cells and pro-duce type 2 responses, so that aggravating the lung inflammation of asthma. Blocking TSLP is capable of in-hibiting the production of Th2 cytokines, thus presents a promising strategy for the treatment of asthma.
