Evaluation of coagulation in vitro with platelet mapping and rapid TEGTM on thrombelastography
10.3760/cma.j.issn.1009-9158.2010.05.017
- VernacularTitle:应用血小板图和快速血栓弹力图评价体外凝血功能
- Author:
Jian LI
;
Yulong CONG
;
Zulan LI
;
Li YANG
;
Chengbin WANG
;
Furong LEI
- Publication Type:Journal Article
- Keywords:
Thrombelastograghy;
Platelet function tests;
Platelet aggregation;
Antibodies,monoclonal;
Immunoglobulin fab fragments;
Heparin
- From:
Chinese Journal of Laboratory Medicine
2010;33(5):453-456
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate performance of PlateletMapping(R) and RapidTEGTM based on thrombelastography by blocking platelet function with Reopro.Methods PlateletMapping was carried out with whole blood from healthy volunteers mixed with Reopro in vitro in a serial of titration.TEG(R) ACT of heparinized blood was tested with Rapid TEG kits.Linearity, repeatability and validity were calculated for two methods.Results MA activated by Kaolin, AA and ADP decreased with the increase of the concentration of Reopro. Inhibition rates (%)for AA and ADP induced aggregation were repeatable in channels and systems.At the Reopro levels of (1-4) × 10-2 mg, inhibition rate increased statistically( AA:27.99% ± 2.8% vs 63.37% ± 0.0% ,t = 21.9, P ＜ 0.01;ADP: 35.9% ± 0.56% vs 91.42% ± 1.14%,t=58.9,P ＜ 0.01 ) after addition of Reopro.Dose-dependent effect relationship could be seen between TEG(R) ACT and heparin;In Rapid TEG assay, measurement repeatability of K, α angle, MA and TEG(R)ACT were all good ( CV ＜ 5% ) except for R.Conclusions PlateletMapping(R) is sensitive to the inhibition of platelet function with good precision with dose-dependent effect.Moreover, Rapid TEG provides analysis of the overall coagulation function besides monitoring heparin therapy.
