Study on CXCR1/CXCR2 antagonist G31P anti-inflammatory reaction mediated by neutrophils
10.3760/cma.j.issn.0254-5101.2010.05.021
- VernacularTitle:CXCR1/CXCR2拮抗剂G31P抗中性粒细胞介导的炎症作用研究
- Author:
Jing WEI
;
Wei LI
;
Wanping SHAO
;
Bing WANG
;
Jing PENG
;
Shiyi WANG
;
Fang LI
- Publication Type:Journal Article
- Keywords:
CXCR1/CXCR2;
IL-8;
Inflammatory response;
Receptor antagonise;
Chemokine
- From:
Chinese Journal of Microbiology and Immunology
2010;30(5):483-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Study on CXCR1/CXCR2 antagonist G31P anti-inflammatory reaction mediated by neutrophils.Methods Detect whether G31P can block chemotaxis of neutrophils induced by human IL-8 and inhibit the release of IL-8 by epithelia of segmental bronchus;establish HEK293 cell line transfected by pcDNA3.0-CXCR1 ,2,4 and detect the chemotaxis of IL-8 for HEK293 ;establish the experi-mental model of pneumonia induced by the P.aeruginosa,take count of the nucleated cells in the bronchoal-veolar lavage fluid(BALF),analyze myeloperoxidase(MPO) of lung tissue and observe the histopathology changing of it.Results G31P can inhibit the chemotaxis for neutrophils and transfected HEK293 cell line,inhibit the A549 releasing of inflammatory mediators;the proportion of neutrophils declines in G31P treat-ment group,pathology examination appears clear discrepancy.Conclusion G31P can block the chemotaxis of chemotactic factor with ELR+ CXC to neutrophils,block the combination of chemotactic factor with its re-ceptor CXCR2,block the CXCR2 on the surface of alveolar epithelia and vascular endothelial cells.Accordingly,neutrophils recruiting to topoinflammation can be prevented.
