Scutellaria baicalensis Inhibits Mast Cell-Mediated Anaphylactic Reactions.
10.11637/kjpa.2010.23.4.217
- Author:
Yun Ho CHOI
1
;
Eui Hyeog HAN
;
Ok Hee CHAI
;
Yun Kyu KIM
;
Hyoung Tae KIM
;
Chang Ho SONG
Author Information
1. Department of Anatomy, Chonbuk National University Medical School, Jeonju, Korea. asch@jbnu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Mast cells;
Anaphylactic reactions;
Scutellaria baicalensis;
Histamine;
Calcium;
cAMP
- MeSH:
Anaphylaxis;
Animals;
Calcium;
Ear;
Histamine;
Histamine Release;
Hypersensitivity, Immediate;
Immunoglobulin E;
Mast Cells;
Medicine, East Asian Traditional;
Methanol;
Mice;
Plasma;
Rats;
Scutellaria;
Scutellaria baicalensis
- From:Korean Journal of Physical Anthropology
2010;23(4):217-227
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mast cells play a critical role in the effector phase of immediate hypersensitivity and allergic diseases. Scutellaria baicalensis is a widely used herb in traditional oriental medicine with anticancer, antiviral, antibacterial and anti-inflammatory properties. However, the roles of Scutellaria baicalensis in mast cell-mediated anaphylactic reactions have not fully been investigated. In this study, we examined the influences of the methanol extract of Scutellaria baicalensis (MESB) on compound 48/80- or anti-dinitrophenyl (DNP) IgE-induced anaphylaxis-like response in vivo. To further prove these in vivo results, the inhibitory effect of MESB on mast cell activation was evaluated, focusing on the histamine release from rat peritoneal mast cells (RPMC). MESB inhibited compound 48/80-induced systemic anaphylaxis-like reaction, plasma histamine release and ear swelling response in mice. MESB also attenuated passive systemic and cutaneous anaphylaxis evoked by anti-DNP IgE. In in vitro experiments, MESB dose-dependently reduced histamine release from RPMC activated by compound 48/80 or anti-DNP IgE. Moreover, compound 48/80-elicited calcium uptake was suppressed in a concentration-dependent manner of MESB. Furthermore, MESB transiently increased the level of intracellular cAMP. From these results, it is suggested that MESB possesses effective anti-anaphylactic activity.
