Regulation of Steroid Thyroid Hormone Receptor 3 (TR3) mRNA Expression by Luteinizing Hormone in Human Early Luteinized Granulosa Cells.
- Author:
Hyun Jeong PARK
1
;
Byung Ryeong KIM
;
Sang Young CHUN
;
Yu Il LEE
Author Information
1. Department of Obstetrics and Gynecology, Chonnam National University Medical School, Gwang-ju, Korea.
- Publication Type:Original Article ; In Vitro
- Keywords:
Steroid thyroid hormone receptor (TR3);
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR);
Luteinizing Hormone (LH);
Ovulation
- MeSH:
Colforsin;
Cyclic AMP-Dependent Protein Kinases;
Cycloheximide;
Dactinomycin;
Female;
Fertilization in Vitro;
Gene Expression;
Gonadotropins;
Granulosa Cells*;
Humans*;
Lutein*;
Luteinizing Hormone*;
Ovulation;
Protein Kinase C;
Receptors, Thyroid Hormone*;
RNA, Messenger*;
Thyroid Gland*
- From:Korean Journal of Obstetrics and Gynecology
2002;45(10):1736-1745
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The present study examined the gonadotropin regulation of TR3 gene expression by luteinizing hormone (LH) in cultured human luteinized granulosa cells. METHODS: TR3 mRNA levels were detected by competitive reverse transcriptase-polymerase chain reaction (RT-PCR) method in cultured human luteinized granulosa cells collected from patients undergoing in vitro fertilization. RESULTS: TR3 transcript was transiently induced by LH, reaching maximum levels 1 hr after stimulation, in a dose-dependent manner. LH-stimulated TR3 expression was abolished by actinomycin D, but was superinduced by cycloheximide. Treatment of luteinized granulosa cells with Rp-cAMP, an inhibitor of protein kinase A, as well as, chelerythrin, an inhibitor of protein kinase C, suppressed LH-stimulated TR3 mRNA levels. In addition, forskolin and TPA mimicked the LH action on the induction of TR3 gene, implying the role of protein kinase A and C activation. CONCLUSION: Taken together, the present study demonstrates that TR3 gene was rapidly and transiently induced by LH in human luteinized granulosa cells. The results imply that TR3 may play a role in ovulation by initiating a cascade of ovulation-specific gene expression in response to LH.
