Trp-Lys-Tyr-Met-Val-Met stimulates phagocytosis via phospho-lipase D-dependent signaling in mouse dendritic cells.
- Author:
Ha Young LEE
1
;
Hyun Kyu KANG
;
Eun Jin JO
;
Jung Im KIM
;
Youl Nam LEE
;
Sang Hwa LEE
;
Yeong Min PARK
;
Sung Ho RYU
;
Jong Young KWAK
;
Yoe Sik BAE
Author Information
1. Medical Research Center for Cancer Molecular Therapy. yoesik@donga.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
chemotactic factors;
dendritic cell;
N-formylmethionine leucyl-phenylalamine;
oligopeptides;
phagocytosis;
phospholipase D;
receptors;
formyl peptid
- MeSH:
1-Butanol/pharmacology;
Animals;
Bone Marrow Cells/cytology/metabolism;
Calcium Signaling/*drug effects;
Cell Death/immunology;
Cell Line;
Communicable Diseases/immunology;
Dendritic Cells/immunology/*metabolism;
Mice;
Neoplasms/immunology;
Oligopeptides/*pharmacology;
Phagocytosis/*drug effects;
Phosphatidic Acids/pharmacology;
Phospholipase D/*metabolism;
Receptors, Formyl Peptide/*metabolism;
Research Support, Non-U.S. Gov't;
tert-Butyl Alcohol/pharmacology
- From:Experimental & Molecular Medicine
2004;36(2):135-144
- CountryRepublic of Korea
- Language:English
-
Abstract:
Dendritic cells (DCs) play a key role in activating the immune response against invading pathogens as well as dying cells or tumors. Although the immune response can be initiated by the phagocytic activity by DCs, the molecular mechanism involved in this process has not been fully investigated. Trp-Lys-Tyr-Met-Val-Met-NH2 (WKYMVM) stimulates the activation of phospholipase D (PLD) via Ca2+ increase and protein kinase C activation in mouse DC cell line, DC2.4. WKYMVM stimulates the phagocytic activity, which is inhibited in the presence of N-butanol but not t-butanol in DC2.4 cells. Furthermore, the addition of phosphatidic acid, an enzymatic product of PLD activity, enhanced the phagocytic activity in DC2.4 cells. Since at least two of formyl peptide receptor (FPR) family (FPR1 and FPR2) are expressed in DC2.4 as well as in mouse bone marrow-derived dendritic cells, this study suggests that the activation of FPR family by WKYMVM stimulates the PLD activity resulting in phagocytic activity in DC2.4 cells.
