Extraction, determination of polyphenol concentration and antioxidant activities of mulberry leaf extract powders
- Author:
Pham Thien Ngoc
- Publication Type:Journal Article
- Keywords:
Mulberry leaf;
Polyphenol content;
Linoleic acid;
Peroxidation.;
Extraction;
Powders;
Antioxidant capacities
- MeSH:
Oxidants;
Morus
- From:Journal of Medical Research
2005;38(5):34-38
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
It has been being some studies on biomedical effects of mulberry leaves in the world. Mulberry trees are widly cultivated in VietNam to take leaves for fed of silkworm. However, there were few studies on extraction, determination and antioxidation of mulberry leaves in VietNam. Objectives: extraction and determination of polyphenol from mulberry leaves. Evaluation of antioxidant capacities of mulberry leaf extract powders. Methods: Mulberry leaf extract powder were extracted from mulberry leaves by five solvens: methanol 100%, methanol 75%, n-hexan 100%, ethylacetat 100%. Polyphenol concentration of the extracts are determined by ferrous sulphate and follin reagent assay. Antioxidant capacities of the powders are based on peroxidation of linoleic acid at 40oC. Results: Among five solvens, the extract by methanol 75% from 50g dried leaves is best. The powder is 1.94g with 2.62% of polyphenol. Polyphenol concentraion of the extract powders are determined by two methods: ferrous sulphate and follin reagent. The result with ferrous sulphate method is more accurate than follin reagent method. The mulberry leave extract powders inhibited linoleic acid peroxidation at the 1h and 12h of reaction (only 15.5% to 42.2% linoleic acid peroxidated) in comparison with control (100% linoleic acid peroxidated). Conclusions: Polyphenol from mulberry leaves was extracted by some solvens. Among them extraction of polyphenol by methanol 75% is best. Polyphenol concentration of extract powders can be dedermined by ferrous sulphat. The extract powders from mulberry leaves exhibited antioxidant capacities in vitro. The extract powder by methanol 75% showed highest antioxidant capacitiy.
