Protective Effect of the Acupuncture for the Mouse Liver Injury Caused by the Administration of Carbon Tetrachloride
10.3777/jjsam.31.315
- VernacularTitle:薬物性肝傷害に対するハリの予防的効果についての実験的超微形態学的研究
- Author:
Nakazo Watari
;
Yasuzo Kurono
;
Tatsuyo Ishigami
;
Yoshie Hiramatsu
;
Shigeru Hori
;
Hironori Nakamura
;
Yoshio Mabuchi
;
Yasuaki Hotta
;
Katsunori Ishigure
- Publication Type:Journal Article
- From:Journal of the Japan Society of Acupuncture and Moxibustion
1982;31(4):315-322
- CountryJapan
- Language:Japanese
-
Abstract:
It is well known that acupuncture has a curative effect for some chronic diseases such as neuralgy or lumbago, if it is administered carefully in terms of the patient. However, the mechanism of acupuncture's effectiveness remains obscure.
In this experiment, using mice of the ICR strain, the protective effect of acupuncture against mouse liver injury was investigated following the administration of carbon tetrachloride.
Experiment I (Fig. 1): One hundred mice were divided into two groups. The first group of animals was administered carbon tetrachloride ip on day 18. The second group was treated a total of six times with acupuncture every three days from the first experimental day to day 18 at the points of Tienshu (ST-25), Feishu (BL-13), Hsinshu (BL-15), Kanshu (BL-18), Pishu (BL-20), Weishu (BL-21), Chimen (LV-14), Chihai (CV-6), Chungwan (CV-12) and Chuchueh (CV-14) (Figs. 3, 4). Fllowing the administration of carbon tetrachloride, the mouse mortality was checked. All of the animals of the first group (carbon tetrachloride administration only) died within 72 hours, but only 84% of the animals of the second group (carbon tetrachloride and acupuncture treatments) died (Fig. 2).
Experiment II (Fig. 1): One hundred and 60 mice were divided into 5 groups. The first group was used for the control (Fig. 5). The second group was injected ip with carbon tetrachloride of 3.47g/Kg BW on day 17. The third group was given a total of 5 acupuncture treatments every three days at the points as mentioned in Experiment I, and carbon tetrachloride was injected as in the second group.
The fourth group was injected a total of 5 times with 0.0004mg levallorphan (an anti-opiate) every three days. The fifth group was given acupuncture treatments like the third group; levallorphan was injected before the acupuncture, and also carbon tetrachloride was injected on day 17 as in the second or third, group.
The animals were sacrificed on day 20, and the liver tissues were treated as usual and then observed by electron microscopy.
The hepatocytes of the second group (carbon tetrachloride only) were heavily injured, and contained, a number of fat droplets, autophagic vacuoles and degenerated cell organelles (Figs. 6, 7, 8, 9). On the other hand, the hepatocytes of the third group (carbon tetrachloride and acupuncture treatments) were almost normal, and without any cell injury (Figs. 10, 11, 12).
The hepatocytes of the fourth group were not injured with levallorphan, suggesting that levallorphan has no cell toxicity (Fig. 13).
The fifth group showed hepatic cell injury similar to the second group (Fig. 14). Levallorphan thus might have an antagonistic action for the acupuncture effectiveness.
In summary, it was concluded that acupuncture has a protective effect against liver injury caused by carbon tetrachloride administration, and prevents not only animal death but also hepatic cell injury. The mechanism of the acupuncture effectiveness is speculated to be as follows. The stimulus of the acupuncture is conducted to the central nervous system and then the central nervous system secretes some neural hormonal substances such as endorphin or enkephalin, which might regulate or accelerate the autonomic nervous system, and then the viscerae functions are accelerated and thus might repair the injuries or dieseases.
In the above-mentioned experiments, levellorphan may have disturbed the secretion or action of endorphin or enkephalin from the central vervous system, which was said to be secreted following the acupuncture treatments, resulting in the ineffectiveness of acupuncture treatments.
Fig. 1. Schedules of the experiments.
Fig. 2. The mortality rate of mice following carbon tetrachloride administration (Experiment I).
Fig. 3. Acupuncture points on the mouse belly.
Fig. 4. Acupuncture points on the mouse back.
Fig. 5. A part of the hepatic cell from a normal mouse. The nucleus (N) and nucleolus (NO) are morphologic
