Anti-Human Cytomegalovirus Activity of Chemical Constituents from Kumazasa Hot Water Extract
10.1625/jcam.7.25
- VernacularTitle:クマザサ含有成分によるヒトサイトメガロウイルスの増殖抑制効果
- Author:
Kazuhiko AKUZAWA
;
Rie YAMADA
;
Changxiao BI
;
Hidetaka SADANARI
;
Keiko MATSUBARA
;
Yuuzou TSUCHIDA
;
Kunitomo WATANABE
;
Masayuki NINOMIYA
;
Mamoru KOKETSU
;
Tsugiya MURAYAMA
- Publication Type:Journal Article
- Keywords:
Human cytomegalovirus;
Sasa senanensis Antiviral activity
- From:Japanese Journal of Complementary and Alternative Medicine
2010;7(1):25-33
- CountryJapan
- Language:Japanese
-
Abstract:
Objective: Anti-human cytomegalovirus (HCMV) activity of 5 compounds, especially tricin, isolated from Kumazasa extract were investigated in the human embryonic lung (HEL) fibroblast cell.
Method: This study examined the effect of tricin on HCMV replication, viral-specific major immediate early (IE) gene expression, and protein synthesis.
Results: Pretreatment of HEL cells before HCMV infection with tricin inhibited viral production in a dose-dependent manner, as well as posttreatment of HCMV-infected HEL cells with tricin. Real-time RT-PCR assay and Western blotting analysis indicated that tricin decreased expression of the HCMV IE gene in virus-infected HEL cell.
Conclusion: These date suggest that the primary mechanism of anti-HCMV activity for tricin, isolated from Kumazasa hot water extract, inhibit IE gene expression.
