Effects of Some Essential Oils on Superoxide Radical Formation in the Reaction Mixture of Riboflavin under UVA Irradiation
10.1625/jcam.6.151
- VernacularTitle:リボフラビン溶液中への ＵＶＡ 照射によるスーパーオキシド生成に対する各種精油（エッセンシャルオイル）の効果
- Author:
Hiroko MORI
;
Hideo IWAHASHI
- Publication Type:Journal Article
- Keywords:
Riboflavin;
Superoxide radical;
Tea tree oil;
ESR (electron spin resonance)
- From:Japanese Journal of Complementary and Alternative Medicine
2009;6(3):151-156
- CountryJapan
- Language:Japanese
-
Abstract:
Objective: The effects of essential oils on the formation of superoxide radicals during UVA (365 nm) irradiation were investigated.
Methods: ESR (electron spin resonance) spin-trapping experiments were performed on a reaction mixture containing 15 μM riboflavin, 0.10 M DMPO (5,5-dimethyl-l-pyrroline-N-oxide), 23 mM phosphate buffer (pH 7.4) and 6.3% (v/v) DMSO (dimethyl sulfoxide) (standard reaction mixture) during UVA (365 nm) irradiation.
Results: On addition of various concentrations of tea tree oil (or lavender super oil or grapefruit oil or peppermint oil or wintergreen oil) to the standard reaction mixture, the ESR peak height decreased dose-dependently.
Conclusion: This result shows that antioxidant activities increase as follows: lavender super oil
