Pilot Study of Antioxidant Mixture (Vitamin E, Pycnogenol and Squalene) in Healthy Smokers: Inhibitory Effect on Oxidative DNA Damage
10.1625/jcam.4.33
- Author:
Satoshi OHNO
;
Yumiko OHNO
;
Nobutaka SUZUKI
;
Nobuhide KAWAGOE
;
Takanari ARAI
;
Masaki INOUE
- Publication Type:Journal Article
- Keywords:
Pycnogenol®
;
Vitamin E;
8-hydroxy-2′
-deoxyguanosine (8-OHdG);
smoking;
clinical trial
- From:Japanese Journal of Complementary and Alternative Medicine
2007;4(1):33-36
- CountryJapan
- Language:English
-
Abstract:
Oxidative stress is considered to contribute to degenerative disease. The urinary excretion of the DNA repair product 8-hydroxy-2′-deoxyguanosine (8-OHdG) is proposed as a noninvasive biomarker of current oxidative stress in vivo. We investigated the effect of an antioxidant mixture on urinary 8-OHdG excretions in 12 otherwise healthy smokers. During the intervention period for 2 weeks, subjects consumed four capsules of PICACE® (Pycnogenol® 15 mg/capsule, Vitamin E; 56.1 mg/capsule, Squalene; 138.9 mg/capsule) per day. On days 0 (pre-internal use), 3, 7, 14, and 44, morning urine samples were collected. The urinary 8-OHdG was measured using high-performance liquid chromatography (HPLC). The urinary 8-OHdG level on day 3 was significantly reduced compared to day 0. The level of 8-OHdG after a washout period for PICACE® (days 44) returned to day 0 baseline. These preliminary data suggest that PICACE® supplements can protect smokers from oxidative stress and possibly reduce disease risk caused by free radicals associated with smoking.
