Ribosome Profiling for Biomarker Discovery
10.1625/jcam.10.1
- VernacularTitle:リボソームプロファイリングのバイオマーカー開発への応用
- Author:
Hiroaki SAKO
;
Katsuhiko SUZUKI
;
Haruko TAKEYAMA
- Publication Type:Journal Article
- Keywords:
ribosome profiling;
post-transcriptional regulation;
mRNA;
gene expression level;
biomarker
- From:Japanese Journal of Complementary and Alternative Medicine
2013;10(1):1-7
- CountryJapan
- Language:Japanese
-
Abstract:
It has been well known that protein level is estimated by the expression level of its mRNA. However, it is also argued that correlation between mRNA abundance and protein levels is weaker than previously thought. Recently a newly developed technique called ribosome profiling has drawn attention as a drastic countermeasure to improve the weak correlation. Here it is discussed that weak association of protein and mRNA levels seen in genome-wide analysis of gene expression such as microarray is attributable to post-transcriptional regulation including translational inhibition. This review further discusses how these issues are resolved by ribosome profiling and also addresses a possibility of biomarker discovery derived from this technique.
