Possible roles of LFA-1 and Fc gamma receptors on the functional immaturities of cord blood polymorphonuclear leukocytes.
10.3349/ymj.1993.34.2.126
- Author:
Nan Ae KIM
1
;
In Hong CHOI
;
Jeon Soo SHIN
;
Sun Kyung PARK
;
Nam Soo KIM
;
Dong Hee CHOI
;
Se Jong KIM
Author Information
1. Department of Microbiology, Yonsei University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Cord blood PMNs;
rosette forming cells;
Fc gamma receptors;
LFA-1
- MeSH:
Antibody-Dependent Cell Cytotoxicity;
Cell Adhesion;
Chemotaxis, Leukocyte;
Fetal Blood/*cytology;
Human;
Lymphocyte Function-Associated Antigen-1/*physiology;
Neutrophils/*physiology;
Receptors, IgG/*physiology;
Rosette Formation
- From:Yonsei Medical Journal
1993;34(2):126-132
- CountryRepublic of Korea
- Language:English
-
Abstract:
The functional immaturity of PMNs is one of the major causes of overwhelming sepsis in newborns. In this study, we observed functions and surface markers of PMNs to investigate what causes the functional immaturity of PMNs in newborns. As results, the percentage of EA rosette forming PMNs (58.5 +/- 15.5%) and the chemotactic movement (0.14 +/- 0.09 mm) of cord blood PMNs were significantly lower than those of adult peripheral blood PMNs (70.8 +/- 9.9%, 0.60 +/- 0.34 mm). Cord blood PMNs showed decreased glass adherence and ADCC activity. The expression of Fc gamma RII or Fc gamma RIII was a little lower than those of adult peripheral blood PMNs, but the expression of Fc gamma RI (43.1 +/- 26.8%) was significantly higher than that of adult peripheral blood PMNs (3.2 +/- 1.8%). There was a significant difference in LFA-1 expression between EA rosette forming PMNs (92.9 +/- 9.1%) and EA rosette non-forming PMNs (25.6 +/- 22.6%). From these results, it is assumed that neonatal PMNs may consist of heterogeneous populations. And the relatively high percentage of EA rosette non-forming PMNs which express a low level of LFA-1 may be responsible for the functional immaturity of cord blood PMNs.
