TNF-alpha upregulates PTEN via NF-kappaB signaling pathways in human leukemic cells.
- Author:
Young Rae LEE
1
;
Hong Nu YU
;
Eun Mi NOH
;
Hyun Jo YOUN
;
Eun Kyung SONG
;
Myung Kwan HAN
;
Chang Sik PARK
;
Byung Soo KIM
;
Young Seok PARK
;
Byung Kwon PARK
;
Sung Ho LEE
;
Jong Suk KIM
Author Information
1. Department of Biochemistry, Chonbuk National University Medical School, Jeonju 561-180, Korea. jsukim@moak.chonbuk.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
leukemia;
NF-kappaB;
PTEN phosphohydrolase;
tumor necrosis factor-alpha
- MeSH:
Up-Regulation/*drug effects;
Tumor Necrosis Factor-alpha/*pharmacology;
Signal Transduction/*drug effects;
PTEN Phosphohydrolase/genetics/*metabolism;
NF-kappa B/genetics/*metabolism;
Leukemia/genetics/*metabolism;
Humans;
Gene Expression;
Cell Line, Tumor
- From:Experimental & Molecular Medicine
2007;39(1):121-127
- CountryRepublic of Korea
- Language:English
-
Abstract:
TNF-alpha plays a variety of biological functions such as apoptosis, inflammation and immunity. PTEN also has various cellular function including cell growth, proliferation, migration and differentiation. Thus, possible relationships between the two molecules are suggested. TNF-alpha has been known to downregulate PTEN via NF-kappaB pathway in the human colon cell line, HT-29. However, here we show the opposite finding that TNF-alpha upregulates PTEN via activation of NF-kappaB in human leukemic cells. TNF-alpha increased PTEN expression at HL-60 cells in a time- and dose-dependent manner, but the response was abolished by disruption of NF-kappaB with p65 anisense phosphorothioate oligonucleotide or pyrrolidine dithiocarbamate. We found that TNF-alpha activated the NF-kappaB pathways, evidenced by the translocation of p65 to the nucleus in TNF-alpha-treated cells. We conclude that TNF-alpha induces upregulation of PTEN expression through NF-kappaB activation in human leukemic cells.
