Kinetin Improves Barrier Function of the Skin by Modulating Keratinocyte Differentiation Markers.
- Author:
Sungkwan AN
1
;
Hwa Jun CHA
;
Jung Min KO
;
Hyunjoo HAN
;
Su Young KIM
;
Kyung Suk KIM
;
Song Jeong LEE
;
In Sook AN
;
Sangwon KIM
;
Hae Jeong YOUN
;
Kyu Joong AHN
;
Soo Yeon KIM
Author Information
- Publication Type:Clinical Trial ; Original Article
- Keywords: Cell culture techniques; Differentiation; Keratinocytes; Kinetin; Skin barrier
- MeSH: Antigens, Differentiation*; Cell Culture Techniques; Epidermis; Humans; In Vitro Techniques; Keratin-1; Keratinocytes*; Kinetin*; Plants; Real-Time Polymerase Chain Reaction; RNA, Messenger; Skin Aging; Skin*; Treatment Outcome; Water
- From:Annals of Dermatology 2017;29(1):6-12
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Kinetin is a plant hormone that regulates growth and differentiation. Keratinocytes, the basic building blocks of the epidermis, function in maintaining the skin barrier. OBJECTIVE: We examined whether kinetin induces skin barrier functions in vitro and in vivo. METHODS: To evaluate the efficacy of kinetin at the cellular level, expression of keratinocyte differentiation markers was assessed. Moreover, we examined the clinical efficacy of kinetin by evaluating skin moisture, transepidermal water loss (TEWL), and skin surface roughness in patients who used kinetin-containing cream. We performed quantitative real-time polymerase chain reaction to measure the expression of keratinocyte differentiation markers in HaCaT cells following treatment. A clinical trial was performed to assess skin moisture, TEWL, and evenness of skin texture in subjects who used kinetin-containing cream for 4 weeks. RESULTS: Kinetin increased involucrin, and keratin 1 mRNA in HaCaT cells. Moreover, use of a kinetin-containing cream improved skin moisture and TEWL while decreasing roughness of skin texture. CONCLUSION: Kinetin induced the expression of keratinocyte differentiation markers, suggesting that it may affect differentiation to improve skin moisture content, TEWL, and other signs of skin aging. Therefore, kinetin is a potential new component for use in cosmetics as an anti-aging agent that improves the barrier function of skin.
