A phenomenon useful for the detection of Salmonella implementing a device from citrus extracts
10.2149/tmh.2008-29
- Author:
Yutaka Midorikawa
;
Paul N Newton
;
Satoshi Nakamura
;
Rattanaphone Phetsouvanh
;
Kaoru Midorikawa
- Publication Type:Journal Article
- Keywords:
Salmonella Proteus Citrobacter identification;
hydrogen sulfide;
citrus;
ascorbic acid;
citric acid;
Laos;
Japan
- From:Tropical Medicine and Health
2009;37(3):115-120
- CountryJapan
- Language:English
-
Abstract:
The effect of lemon slices, as well as ascorbic and citric acid impregnated paper discs, on the growth of ten non-typhoidal Salmonella, six Citrobacter freundii and four Proteus mirabilis species on Desoxycholate Hydrogen Sulfide Lactose (DHL) agar were examined in comparison to controls without fruit slices or paper discs applied. After 24 h incubation, thick black rings were observed around fruit slices and impregnated discs growing on non-typhoidal Salmonella serovars and not around the other species. We named this the “MY Phenomenon”. We propose that the phenomenon can be used as a rapid, simple and inexpensive screening test that distinguishes non-typhoidal Salmonella species from other enterobacteriaceae in stool samples.
