Comparison between UV and HPLC methods in the dosage of pharmaceutical products containing a mixture of 2 components amoxicillin and clavulanic acid
- Author:
Trinh Thi Thu Loan
- Publication Type:Journal Article
- Keywords:
Pharmaceutical product;
Amoxicillin;
Clavulanic acid;
Dosage;
UV method;
HPLC method;
Comparison
- MeSH:
Pharmaceutical Preparations;
Amoxicillin;
Clavulanic Acid
- From:Pharmaceutical Journal
2003;0(6):29-32
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
UV - spectrophotometric (UV) and high performance liquid chromatographic methods (HPLC) were described in determining amoxicillin and clavulanic acid in pharmaceutical preparations. Spectrophotometrically, amoxicillin was determined by measuring the absorbance values at 320 nm in buffer-CuS04 solution (pH 5.2) for amoxicillin and at 313 nm in imidazol solution (pH 6.8) for clavulanic acid. Beer's Law was obeyed in range 12 - 32mg. ml-1 for amoxicillin and 12 - 22 mg. ml-1 for clavulanic acid. The simultaneous HPLC method depended upon using a reserved phase RP18 column at ambient temperature with a mobile phase consisting of methanol - phosphate solution pH 4.4 (4:96) at a flow rate 1ml.min-1. Quantitation was achieved by UV detection at 220 nm. Calibration curve was linear over the concentration range 50-150 mg. ml-1 for amoxicillin and 15-35 mg. ml-1 for clavulanic acid. Both spectrophotometry and HPLC method showed good linear correlation, precision and accuracy. The obtained results from spectrophotometry and HPLC method showed no significant difference. Both methods were successfully applied to the assay of commercial tablets. The procedures were rapid, simple and suitable for quality control applications
