Effects of yperthermia on Human Platelets. Flow cytometric analysis on the formation of platelet-derived microparticles and the expression of CD62 antigen on the platelet surface.
- VernacularTitle:温熱の血小板に与える影響 特に血小板由来マイクロパーティクルの生成と血小板膜上におけるＣＤ６２抗原の発現について
- Author:
Hitoshi TAKE
;
Kazuo KUBOTA
;
Kousei TAMURA
;
Hitoshi KURABAYASHI
;
Jun'ichi TAMURA
;
Takuo SHIRAKURA
;
Mayumi MORI
- Publication Type:Journal Article
- Keywords:
Hyperthermia;
Platelet-derived microparticle;
CD62;
Flow cytometry
- From:The Journal of The Japanese Society of Balneology, Climatology and Physical Medicine
1995;58(4):213-217
- CountryJapan
- Language:Japanese
-
Abstract:
Effects of hyperthermia on the formation of platelet-derived microparticles (MP) and the expression of surface CD62 antigen were examined in normal human platelets. Venous blood from healthy subjects, anticoagulated with 1 volume of 3.8% sodium citrate, was heated at 37°C (control), 42°C and 47°C for 15 minutes. Then 2μl of each sample was incubated with FITC or PE-conjugated anti-human CD42b or CD62 antibodies, and assayed for MP and CD62 by flow cytometry. The percentage of MP after the incubation was not significantly different from that before the incubation nor that of control (9.9±0.6% before incubation, 10.2±0.6% at 37°C, 10.8±0.4% at 42°C and 10.3±0.3% at 47°C), CD62 positive-platelets slightly increased after the incubation, but no significant differences were observed between the control value and the values at 42°C and 47°C (1.6±0.3% at 37°C, 1.9±0.5% at 42°C and 1.7±0.3% at 47°C). These data suggest that hyperthermia has only a weak stimulatory effect on platelets and is unable to induce MP formation.
