Myotube-like structure in aged mouse.
- Author:
KAZUYUKI OKA
;
SHINNOSUKE TAKAYANAGI
;
MASUO MURO
- Publication Type:Journal Article
- Keywords:
Skeletal muscle;
Chained nuclei;
DNA fragmentation;
Aging
- From:Japanese Journal of Physical Fitness and Sports Medicine
2002;51(4):357-366
- CountryJapan
- Language:English
-
Abstract:
A small amount of the myotube-like structures (chained nuclei) are present in skeletal muscle independently of disease. These experiments show that the incidence of the myotube-like structure increases with the advance of age in mice. Moreover, it is shown that the DNA in the myotube-like structure is not fragmented. That is, the myotube-like structures were TUNEL (Terminal deoxynuc-leotidyl transferase (TdT) -mediated dUTP-biotin Nick-End labeling) negative. Moreover, we detected the polyadenylated mRNA around the myotube-like structure by using an in situ hybridization technique. This suggests that the myotube-like structure has transcription activity.
This report indicates that the myotube-like structure is a useful indicator of aging in skeletal muscle.
