Changes in the Expression Levels of Stress Proteins in Rat Skeletal Muscles Due to Heat Stress Exposure.
- VernacularTitle:ラット骨格筋への熱負荷にともなう熱ショックタンパク質の発現変化
- Author:
YASUHARU OISHI
;
YOSHIYUKI FUKUOKA
;
AKIHIKO ISHIHARA
;
YOSHINOBU OHIRA
;
KOUHACHI TANIGUCHI
;
HISAHIRO MATSUMOTO
- Publication Type:Journal Article
- Keywords:
heat shock protein;
heat stress;
Western blot;
soleus;
plantaris;
rat
- From:Japanese Journal of Physical Fitness and Sports Medicine
2001;50(2):193-200
- CountryJapan
- Language:Japanese
-
Abstract:
The expression levels of heat shock proteins after heat stress on rat slow soleus and fast plantaris muscles were examined and compared during a recovery period following 1 h of heat stress. The left hindlimbs of adult male Wistar rats (n=15) were carefully inserted into a stainless steel can and subjected to heat stress for 1 h by raising the air temperature inside the steel can to 54-58t with a flexible heater so as to bring the muscle temperature up to 42°C. The muscles of the contralateral right hindlimb served as the control. The expression levels of HSP 60, HSP 72, and HSC 73 were analyzed by Western blotting after 0, 2, and 4 h of recovery following 1 h of heat stress. In the soleus muscle, all of the HSP levels analyzed were significantly increased during 0-4 h of recovery. On the other hand, heat stress had no effect on the expression levels of HSPs, except HSP 60, in the plantaris muscle during recovery after 1 h of heat stress. These results suggest that the slow soleus muscle has a higher ablility to respond quickly to heat stress than the fast plantaris muscle.
