Effects of hindlimb suspension on patterns of myosin isoforms in rat soleus muscle.
- VernacularTitle:尾部懸垂によるラットヒラメ筋におけるミオシンアイソフォームの分布の変化
- Author:
TADASHI OKUMOTO
;
AYAKO SAITOH
;
SHIGERU KATSUTA
;
SHIGEMITSU NIIHATA
;
KUNIO KIKUCHI
;
MASANOBU WADA
- Publication Type:Journal Article
- Keywords:
hindlimb suspesion;
isomyosins;
myosin heavy chain isoforms;
myosin light chain isoforms
- From:Japanese Journal of Physical Fitness and Sports Medicine
1997;46(3):279-287
- CountryJapan
- Language:Japanese
-
Abstract:
Using several electrophoretic techniques, this study examined the effects of 3 weeks hindlimb suspension on the patterns of isomyosins, myosin heavy chain (HC) isoforms and myosin light chain (LC) isoforms in the soleus muscle of the rat. The suspended soleus showed a shift in the HC isoform distribution with a marked increase in fast HC isoforms and a commensurate decrease in HCI. In addition, the change in the fast HC isoforms consisted of the expression of HCIId and HC IIb absent in the normal soleus. In contrast to HC isoforms, suspension did not lead to appreciable changes in LC isoform distribution. Analyses of electrophoresis under nondenaturing conditions demonstrated that the normal soleus expressing HCI and HCIIa isoforms contained two isomyooins. Although, of the two isomyosins observed in the normal soleus, the faster migrating band most likely represented the HCIIa-based one (FMas), its mobility was not identical with that of the HCIIa-based isomyosin (FMaf) found in fast-twitch muscles, migrating in the order FMaf>FMas. FMas was designated as intermediate isomyosin (IM) . Some of the suspended soleus contained slow isomyosin (SM) and IM whereas the others comprised FM 3 and/or FM 2 as well as SM and IM. In spite of the expression of HCIIb and HCIId in the suspended soleus, FM 3 and FM 2 observed in these muscles exhibited distinct mobilities from either HCIId-based or HCIIb-based isomyosins comprised in fast-twitch muscles. These results suggest that some of newly expressed HCIId and/or HCIIb isoforms in the suspended soleus are associated with not only fast but also slow LC isoforms and function as a constitutive element of the myosin molecule.