The combined effects of stressful exercise and sleep disturbance on rat. Part 1. Combined effects started from childhood on cells in bronchoalveolar lavage fluids.
- VernacularTitle:強制運動と睡眠妨害の組み合わせがラットに及ぼす影響 第１報 気管支肺胞洗浄液中細胞に及ぼす幼若期からの影響
- Author:
TAKASHI KUMAE
;
HATSUKO ARAKAWA
;
IWAO UCHIYAMA
- Publication Type:Journal Article
- Keywords:
Rat;
Chronic Fatigue;
Cellular Immunity;
Alveolar Macrophage;
Chemiluminescence
- From:Japanese Journal of Physical Fitness and Sports Medicine
1996;45(2):301-310
- CountryJapan
- Language:Japanese
-
Abstract:
Phagocytic cells, polymorphonuclear leucocytes and macrophage, play an important role in a non-specific cellular immunity which is first defense line for infectious disease. The purpose of this paper is to clarify combined effects of stressful exercise and sleep disturbance on rat non-specific cellular immunity represented by yields of cells from bronchoalveolar lavage and superoxide generating capacity of alveolar macrophage.
Male Wistar rats ( 5 weeks old) were divided into following 4 groups. 1) Training group; exercised on a treadmill at a belt speed of 35 m/min for 45 min/day, 2) Jet Lag group; noninvasive sleep disturbance by the shifted day/night time every 2 week interval, 3) Training + Jet Lag (T+ J) group ; exercised on a treadmill with sleep disturbance, that we assumed one of chronic fatigue model in this paper, and 4) Control group ; set sedentary condition.
After 6 weeks of the experiment, 2 groups were newly selected from T + J group for the examination of rest effects on the chronic fatigue model rats. And to examine the combined effects of stressful exercise and sleep disturbance on matured rats (11 weeks old), Acute Training + Jet Lag (AT+J) group was selected from the Control group.
Rats were sacrificed at 11 and 17 weeks old. T + J group seemed to be able to adapt first 6 weeks but negative effects were found out at 17 weeks old. Same phenomena were also recognized in AT + J group. These results suggest that the chronic fatigue model rats may easily catch the infectious disease and there are possibility that both stress, stressful exercise and sleep disturbance, negatively affects the non-specific cellular immunity, especially after maturation.
