Analysis of human urinary proteins after physical exercise by two-dimensional electrophoresis under non-denaturing conditions.
- VernacularTitle:身体運動後の尿タンパク質の未変性 ２次元電気泳動法による分析
- Author:
TAKUMITSU HIRO
;
TATSUKI INOUE
;
TORU TOMIOKA
;
KOICHI HIROTA
;
TSUYOKI KADOFUKU
;
YOSHIAKI MAKINO
;
TSUNEO SATO
;
MINORU TAKEDA
- Publication Type:Journal Article
- Keywords:
physical exercise;
urinary protein;
two-dimensional electrophoresis
- From:Japanese Journal of Physical Fitness and Sports Medicine
1993;42(2):138-144
- CountryJapan
- Language:Japanese
-
Abstract:
A study was conducted to investigate the changes in human urinary proteins caused by physical exercise. Nine subjects (male middle-distance runners, 19-21 years) were loaded physical exercise with a 10km-run, and quantitative and qualitative changes in urinary proteins were examined using various techniques including polyacrylamide gel electrophoresis, immunoelectrophoresis and two-dimensional electrophoresis. Total protein concentrations in urine from all subjects before exercise were very low (0.68-2.56mg/dl), but those were increased remarkably after exercise. Few proteins except for albumin were detected in urine before run by polyacrylamide gel electrophoresis and immunoelectrophoresis, however, a few proteins were observed after exercise in addition to a marked increase of albumin. Several proteins which were not observed in serum could also be detected in urine after exercise by two-dimensional electrophoresis under non-denaturing condition. It is considered that two-dimensional electrophoresis is a very powerful technique for analyzing dilute and complicated protein mixture in biological fluids such as urine.
