Histochemical and biochemical studies on the effect of exercise on the skeletal muscle fibers in rats.
- VernacularTitle:ラット骨格筋線維のトレーニング効果に関する研究 組織化学的，生化学的手法を用いての検討
- Author:
HIROAKI TAKEKURA
;
HIROYUKI TANAKA
;
MITSUTSUGU ONO
;
NORIKATSU KASUGA
- Publication Type:Journal Article
- Keywords:
Skeletal muscle fiber;
Fiber type composition;
Enzyme activity
- From:Japanese Journal of Physical Fitness and Sports Medicine
1985;34(5):276-283
- CountryJapan
- Language:Japanese
-
Abstract:
The inter of this study was to observe the effects of training on histochemical and biochemical properties in the skeletal muscle fibers.
Wistar strain albino rats were assigned to endurance (E), sprint (S) and control (C) group at 4 weeks old. Animals of trained groups were trained by treadmill for 12 weeks. After training, all animals were sacrificed and M. Soleus (SOL) and M. Extensor Digitorum Longus (EDL) were taken out.
Using histochemical staining methods for Hematoxylin and Eosin, Actomyosin ATPase and Succinate Dehydrogenase (SDH), area and composition of the three muscle fiber types were examined. Furthermore, the chemical properties of glycolytic and oxidative enzyme activities were examined.
The results obtained were as follows:
1. The E and S trained groups, respectivelly, showed lower increase of body weight than the control group.
2. Hypertrophy of all fiber types in EDL were observed in the both trained groups. Area of SO fibers in SOL were also increased in the E group as compared to the C group.
3. The differences observed for fiber composition among the three groups. The rate of FOG fiber in SOL decreased significantly in the both trained groups as compared to the C group. The rate of SO fiber in both muscles were slightly higer for the E group relative to the C Group, but these differences were no significant. However, in the E group significant increase was observed for oxidative enzyme activity. The Phosphofructo-kinase activity in EDL of the S group was significantly higher in comparison to the other groups.
These results suggested that there is the discrepancy between histochemical proper-ties and biochemical properties in the effects of training on skeletal muscle fibers and that the necessity of histochemical and biochemical analysis.
