Study on dosage of amoxicilline and clavulanic acid in human serum by HPLC method
- Author:
Trinh Thi Thu Loan
- Publication Type:Journal Article
- Keywords:
HPLC method
- MeSH:
Amoxicillin;
Clavulanic Acid;
Serum;
Chromatography, High Pressure Liquid
- From:Pharmaceutical Journal
2005;353(9):23-26
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
A simple, rapid, sensitive, precise and accurate high-performance liquid chromatographic (HPLC) method with ultraviolet detection at wavelength of 205nm has been validated for the simultaneous determination of amoxicillin and clavulanic acid in human serum. Serum samples were deproteinized with methanol. The separation of 2 obtained compounds was achieved using a reversed phase C8 column and a mobile phase, consisting of acetonitrile-phosphate solution. The calibration curves were linear over the concentration range of 0.625 – 10 g.ml-1 for amoxicillin and 0.3125 – 5 g.ml-1 for clavulanic acid. The quantitative limits are 0.625 and 0.3125 g.ml-1 for amoxicillin and clavulanic acid. Analytical recoveries from human serum ranged from 93 to 96% for both components. This method was fully validated. It allows the simultaneous assay of amoxicillin and clavulanic acid in biomedical applications. This method has been successfully applied to a pilot pharmacokinetic study in 3 healthy volunteers after a single oral administration of amoxicillin and clavulanic acid combination
