Establishment of HPLC Method for Quantification of L-Tetrahydropalmatine in Stephanias
- Author:
Pham Thi Duyen
- Publication Type:Journal Article
- Keywords:
Stephanias
- MeSH:
Chromatography, High Pressure Liquid;
Berberine Alkaloids
- From:Journal of Medicinal Materials - Hanoi
2003;8(5):131-135
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
An HPLC method has been established to determine l-tetrahydropalmatine (rotundine) extracted by chloroform from powders of Stephania viridiflavens, S. dielsiana and Stephania sp. (Menispermaceae). The technique was carried out on an RP-18 (250 x 4.0 mm; 5 m) column with a diode array detector or UV detector at = 283 nm, using a mixture of 0.05 M potassium dihydrogen phosphate, acetonitrile and triethylamine (62.5 : 37.5 : 0.5) adjusted to pH 4.0 by concentrated phosphoric acid as a mobile phase. The HPLC method proved accurate, specise and sensitive. The results show that differences exist in the content of l-tetrahydropalmatine between species.
