Sasa borealis extract exerts an antidiabetic effect via activation of the AMP-activated protein kinase.
- Author:
Jung Soo NAM
1
;
Hee Jin CHUNG
;
Min Kyung JANG
;
In Ah JUNG
;
Seong Ha PARK
;
Su In CHO
;
Myeong Ho JUNG
Author Information
- Publication Type:Original Article
- Keywords: Antidiabetic effect; AMP-activated protein kinase; Sasa borealis; insulin signaling
- MeSH: AMP-Activated Protein Kinases; Animals; Blood Glucose; Glucose; Hep G2 Cells; Insulin; Mice; Muscle, Skeletal; Phosphorylation; PPAR alpha; Sasa
- From:Nutrition Research and Practice 2013;7(1):15-21
- CountryRepublic of Korea
- Language:English
- Abstract: Leaf of Sasa borealis, a species of bamboo, has been reported to exhibit anti-hyperglycemic effect. However, its antidiabetic mechanism is not fully understood. In this study, we examined whether an extract of S. borealis activates AMP-activated protein kinase (AMPK) and exerts anti-hyperglycemic effects. Treatment with the S. borealis extract increased insulin signaling and phosphorylation of AMPK and stimulated the expression of its downstream targets, including PPARalpha, ACO, and CPT-1 in C2C12 cells and PPARalpha in HepG2 cells. However, inhibition of AMPK activation attenuated insulin signaling and prevented the stimulation of AMPK target genes. The S. borealis extract increased glucose uptake in C2C12 cells and suppressed expression of the gluconeogenic gene, PEPCK in HepG2 cells. The extract significantly reduced blood glucose and triglyceride levels in STZ-induced diabetic mice. The extract enhanced AMPK phosphorylation and increased Glut-4 expression in the skeletal muscle of the mice. These findings demonstrated that the S. borealis extract exerts its anti-hyperglycemic effect through activation of AMPK and enhancement of insulin signaling.
