Quantitative determination of L-tetrahydropalmatine (Rotundine) in Balok pills by HPLCL
- Author:
Pham Thi Duyen
- Publication Type:Journal Article
- Keywords:
L-tetrahydropalmatine
- MeSH:
Chromatography, High Pressure Liquid;
Berberine Alkaloids
- From:Journal of Medicinal Materials - Hanoi
2003;8(2):70-73
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
One of the constituents of the oriental traditional pill named Balok was Tuber Stephaniae rotundae, the active principle of which was rotundine. The test solutions used for the analysis of rotundine were standardized after ISO/IEC 17027 and GLP, the equipment was adjusted after ISO/IEC 17025. The proposed HPLC technique was proved to be appropriate and stable for the quantitative determination of rotundine. The examined concentration showed the linear correlation between the rotundine concentration and the pic surface. The reproducibility of the method was high, with RSD = 0.98%. The accuracy rate of the method was 100.01%, with RSD = 2.0%.
