Suppressive Effect of Herbal Medicine Dokkatsu-kisei-to on Mouse Type II Collagen-induced Arthritis.
10.3937/kampomed.54.763
- VernacularTitle:マウスコラーゲン関節炎に対する独活寄生湯の影響
- Author:
Toshimitsu SOHMA
;
Morihiro KOGURE
;
Naomi KOSHIISHI
;
Hideshi IKEMOTO
;
Takako KASAHARA
;
Tadashi HISAMITSU
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis model;
mouse collagen arthritis;
Dokkatsu-kisei-to;
prednisolone;
arthritis score;
type II collagen
- From:Kampo Medicine
2003;54(4):763-771
- CountryJapan
- Language:Japanese
-
Abstract:
Collagen-induced arthritis (CIA) in DBA/1J mice is an autoimmune model driven by the combination of humoral and cellular immunity against cartilage type II collagen. It has been proven to be a useful model for human rheumatoid arthritis (RA). This study was designed to obtain experimental evidence of Kampo medicine Dokkatsu-kisei-to (DKT) in the treatment of RA by using mouse CIA. As positive control, prednisolone was given. The significant suppressive effect of DKT as measured by arthritis score persisted upon cessation of treatment on day 42 and remained until the termination of the experiment on day 63. The amelioration of inflammatory articular lesions was confirmed by radiological and histo-pathological findings. We also observed significantly decreased concentrations of serum TNF-α on day 28 and levels of serum IgG antibody against bovine type II collagen on day 49. These results suggest that DKT has important effects on immuno-inflammatory response and may be useful in the prevention of the onset and severity of arthritis.
