Studies on Diagnostic Methods to Detect Drug Allergies to Kampo Medicines. (I): Preliminary Analysis of Antigens from Crude Drugs and Antigen-Specific Antibodies using Shosaiko-to Extracts.
10.3937/kampomed.46.417
- VernacularTitle:漢方薬アレルギーの診断法に関する研究 （Ｉ） 小柴胡湯を用いた生薬抗原と抗原特異抗体の検出法の検討
- Author:
Kazunori FUKUDA
;
Rie SUZUKI
;
Yasuhiro KOMATSU
- Publication Type:Journal Article
- Keywords:
Drug allergies;
antigens;
antigen-specific antibodies;
Kampo medicines;
crude drugs
- From:Kampo Medicine
1995;46(3):417-426
- CountryJapan
- Language:Japanese
-
Abstract:
Recently, there has been an increase in reports of allergic reactions to Kampo medicines. In order to elucidate the mechanisms of these so-called Kampo allergies, suitable methods are necessary to detect allergens in crude drugs and allergenspecific antibodies in sensitized individuals. In this study, methodology and sensitivity were studied using enzyme-linked immunosorbent assay and Western blot analysis. The antigens used were haptenized baicalin and high-molecular-weight components of Ginseng Radix, both derived from Shosaiko-to extract.
Attempts were also made to further identify antigens from Shosaiko-to and detect antigenspecific antibodies in immunized rabbits.
