Simultaneous Determination of Berberine and Palmatine in “Huong Lien Hoan” by HPLC
- Author:
Nguyen Thi Phuong Thao
- Publication Type:Journal Article
- Keywords:
Berberine, Palmatine
- MeSH:
Berberine;
Medicine, Traditional;
Chromatography, High Pressure Liquid;
Biochemistry;
Berberine Alkaloids
- From:Journal of Medicinal Materials - Hanoi
2003;8(2):45-49
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Berberine and Palmatine were extracted from the powdered Huong Lien pills with a mixture of methanol and hydrochloric acid (100:1) and subjected to HPLC to simultaneously determine the alkaloids. The HPLC technique was carried out on a Lichrosorb RP-8 (10 m, 250x4 mm) column using a UV detector at =345 nm and a mixture of 3,4g potassium dihydrogen phosphate and 1,7g sodium lauryl sulfate dissolved in 1000 ml of water-acetonitrile (1:1) as a mobile phase. The experimental results proved that the proposed HPLC method was rapid, specific, accurate and precise.
