Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid.
10.4062/biomolther.2015.010
- Author:
Jin Fu PENG
1
;
Li LIU
;
Cheng Xian GUO
;
Shi Kun LIU
;
Xiao Ping CHEN
;
Li Hua HUANG
;
Hong XIANG
;
Zhi Jun HUANG
;
Hong YUAN
;
Guo Ping YANG
Author Information
1. Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410000, China.
- Publication Type:Original Article
- Keywords:
miR-511;
OATP1B1;
SCLO1B1;
Expression;
Free fatty acid
- MeSH:
Computational Biology;
Fatty Acids, Nonesterified;
Humans;
Liver;
Luciferases;
MicroRNAs;
RNA, Untranslated
- From:Biomolecules & Therapeutics
2015;23(5):400-406
- CountryRepublic of Korea
- Language:English
-
Abstract:
MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.
