Imperatorin Suppresses Degranulation and Eicosanoid Generation in Activated Bone Marrow-Derived Mast Cells.
10.4062/biomolther.2015.023
- Author:
Kyu Tae JEONG
1
;
Eujin LEE
;
Na Young PARK
;
Sun Gun KIM
;
Hyo Hyun PARK
;
Jiean LEE
;
Youn Ju LEE
;
Eunkyung LEE
Author Information
1. Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-210, Republic of Korea. eklee@ynu.ac.kr
- Publication Type:Original Article
- Keywords:
Leukotriene C4;
Prostaglandin D2;
Cytosolic phospholipase A2;
Mitogen-activated protein kinases;
Phospholipase Cgamma1
- MeSH:
Animals;
Calcium;
Cytosol;
Eicosanoids;
Inflammation;
Leukotriene C4;
Mast Cells*;
Mice;
Mitogen-Activated Protein Kinases;
Phospholipases;
Prostaglandin D2;
Protein Kinases
- From:Biomolecules & Therapeutics
2015;23(5):421-427
- CountryRepublic of Korea
- Language:English
-
Abstract:
Imperatorin has been known to exert many biological functions including anti-inflammatory activity. In this study, we investigated the inhibitory effects of imperatorin on the production of inflammatory mediators in mouse bone marrow-derived mast cells (BMMC). Imperatorin inhibited degranulation and the generation of eicosanoids (leukotriene C4 (LTC4) and prostaglandin D2 (PGD2)) in IgE/antigen (Ag)-stimulated BMMC. To elucidate the molecular mechanism involved in this process, we investigated the effect of imperatorin on intracellular signaling in BMMC. Biochemical analyses of the IgE/Ag-mediated signaling pathway demonstrated that imperatorin dramatically attenuated degranulation and the production of 5-lipoxygenase-dependent LTC4 and cyclooxygenase-2-dependent PGD2 through the inhibition of intracellular calcium influx/phospholipase Cgamma1, cytosolic phospholipase A2/mitogen-activated protein kinases and/or nuclear factor-kappaB pathways in BMMC. These results suggest that the effects of imperatorin on inhibition of degranulation and eicosanoid generation through the suppression of multiple steps of IgE/Ag-mediated signaling pathways would be beneficial for the prevention of allergic inflammation.
