Gastroprotective Effect of Cochinchina momordica Seed Extract in Nonsteroidal Anti-Inflammatory Drug-Induced Acute Gastric Damage in a Rat Model.
- Author:
Ji Hwan LIM
1
;
Joo Hyun KIM
;
Nayoung KIM
;
Byoung Hwan LEE
;
Pyoung Ju SEO
;
Jung Mook KANG
;
So Young JO
;
Ji Hyun PARK
;
Ryoung Hee NAM
;
Hyun CHANG
;
Jin Won KWON
;
Dong Ho LEE
Author Information
1. Department of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea. nayoungkim49@empal.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cochinchina momordica;
Gastroprotection;
Anti-inflammatory agents, non-steroidal;
Arachidonate 5-lipoxygenase;
Phospholipases A2, cytosolic
- MeSH:
Animals;
Anti-Inflammatory Agents, Non-Steroidal/adverse effects;
Arachidonate 5-Lipoxygenase/drug effects;
Calcitonin Gene-Related Peptide/drug effects;
Cyclooxygenase 1/drug effects;
Cyclooxygenase 2/drug effects;
Disease Models, Animal;
Gastric Mucosa/chemistry/drug effects;
Group IV Phospholipases A2/drug effects;
Male;
Momordica/*chemistry;
Peroxidase/drug effects;
Plant Extracts/*pharmacology;
Rats;
Rats, Sprague-Dawley;
Seeds/*chemistry;
Stomach Ulcer/chemically induced/*prevention & control;
Treatment Outcome
- From:Gut and Liver
2014;8(1):49-57
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: The major compounds of Cochinchina momordica seed extract (SK-MS10) include momordica saponins. We report that the gastroprotective effect of SK-MS10 in an ethanol-induced gastric damage rat model is mediated by suppressing proinflammatory cytokines and downregulating cytosolic phospholipase A2 (cPLA2), 5-lipoxygenase (5-LOX), and the activation of calcitonin gene-related peptide. In this study, we evaluated the gastroprotective effects of SK-MS10 in the nonsteroidal anti-inflammatory drug (NSAID)-induced gastric damage rat model. METHODS: The pretreatment effect of SK-MS10 was evaluated in the NSAID-induced gastric damage rat model using aspirin, indomethacin, and diclofenac in 7-week-old rats. Gastric damage was evaluated based on the gross ulcer index by gastroenterologists, and the damage area (%) was measured using the MetaMorph 7.0 video image analysis system. Myeloperoxidase (MPO) was measured by enzyme-linked immunosorbent assay, and Western blotting was used to analyze the levels of cyclooxygenase (COX)-1, COX-2, cPLA2, and 5-LOX. RESULTS: All NSAIDs induced gastric damage based on the gross ulcer index and damage area (p<0.05). Gastric damage was significantly attenuated by SK-MS10 pretreatment compared with NSAID treatment alone (p<0.05). The SK-MS10 pretreatment group exhibited lower MPO levels than the diclofenac group. The expression of cPLA2 and 5-LOX was decreased by SK-MS10 pretreatment in each of the three NSAID treatment groups. CONCLUSIONS: SK-MS10 exhibited a gastroprotective effect against NSAID-induced acute gastric damage in rats. However, its protective mechanism may be different across the three types of NSAID-induced gastric damage models in rats.
